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KMID : 0358320080490050432
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Korean Journal of Urology
2008 Volume.49 No. 5 p.432 ~ p.438
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Effects of Mesenchymal Stem Cells on Stress Incontinence in a Rat Model
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Son Kyung-Chul
Kim Sun-Ouck
Lee Jae-Hyuk
Kwon Dong-Deuk
Joo Soo-Yeon
Ahn Young-Keun
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Abstract
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Purpose: Several study trials have used stem cells to treat stress incontinence in an animal model. In this study, we compared injecting either periurethral mesenchymal stem cells(MSC) or normal saline(C) to increase the leak point pressure(LPP) and closing pressure(CP) in a rat model of stress urinary incontinence.
Materials and Methods: Sprague Dawley rats(250g each, 12 weeks old) were divided into the MSC group(n=5) and group C(n=5). They were anesthetized and the pudendal nerve was transected bilaterally via a ventral incision in order to denervate the external urethral sphincter. The MSCs were obtained from both femurs of Sprague Dawley rats(150g each, 6 weeks, n=10). After 1 week, the MSCs were stained by 4’-6-diamidino- 2-phenylindole(DAPI), which was injected into both sites of the proximal external urethra(n=1.5x106). At 3 weeks after injection, cystometry was performed and this was followed by cord transection at the T9-10 level with the rat under anesthesia. Visually identified LPP and CP measurements were evaluated with using a vertical tilt/intravesical pressure clamp. The urethral tissues of the rats were harvested for histology.
Results: Both the LPP and CP measurements were significantly higher in the MSC group when compared with that of the C group(p＜0.05). The mean LPP of the MSC group and group C was 42.3±2.1cmH2O and 25.8± 1.7cmH2O, respectively. The mean CP of the MSC group and group C was 31.7±2.5cmH2O and 21.3±1.1cmH2O, respectively. The existence of DAPI-stained MSCs in the injected periurethral tissue was verified by histology after the completion of the study.
Conclusions: Injection of MSCs into the periurethal tissue after transection of the bilateral pudendal nerve in rats led to an increase in the LPP and CP. This finding suggests that MSCs can be used as one of the potentially effective cell therapies for stress urinary incontinence.
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KEYWORD
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Urinary incontinence, Mesenchymal stem cells, Urodynamics
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